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. 2009 Apr 13;4(4):e5185. doi: 10.1371/journal.pone.0005185

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Aranko et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) SDS-PAGE analysis of the time course from the protein ligation reaction of nSH3 and cSH3 in the presence of 0.5 mM TCEP. Lane 1, 0 min after the mixing; lane 2, 3 min; lane 3, 10 min; lane 4, 30 min; lane 5, 1 hour; lane 6, 3 hours; lane 7, 22 hours. (b) Kinetic analysis of the protein ligation from the SDS-PAGE.