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Time courses and kinetic analysis of protein ligation in the presence of (a) 0.5 mM TCEP or (b) 50 mM DTT. SDS-PAGE: lane 1, 0 min after the mixing; lane 2, 3 min; lane 3, 10 min; lane 4, 30 min; lane 5, 1 hour; lane 6, 3 hours; lane 7, 24 hours.
