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. 2009 Apr 13;4(4):e5185. doi: 10.1371/journal.pone.0005185

Table 1. The final yields of the protein ligation by protein trans-splicing.

Intein N-extein C-extein Yield (%) with 50 mM DTT Yield (%) with 0.5 mM TCEP
NpuDnaE-IntN102/C36 (wild-type) nSH3 cSH3 n.d. n.d.
GB1 cSH3 n.d. n.d.
nSH3 GB1 77±10 61±8
NpuDnaE-IntN123/C15 nSH3 cSH3 27±10 50±7
Smt3 GB1 9±3 65±7

n.d., not detectable.