Table 1. The final yields of the protein ligation by protein trans-splicing.
Intein | N-extein | C-extein | Yield (%) with 50 mM DTT | Yield (%) with 0.5 mM TCEP |
NpuDnaE-IntN102/C36 (wild-type) | nSH3 | cSH3 | n.d. | n.d. |
GB1 | cSH3 | n.d. | n.d. | |
nSH3 | GB1 | 77±10 | 61±8 | |
NpuDnaE-IntN123/C15 | nSH3 | cSH3 | 27±10 | 50±7 |
Smt3 | GB1 | 9±3 | 65±7 |
n.d., not detectable.