TABLE III.
Endogenous channel RIV fingerprint is evident in SLC26A9 + ΔF508CFTR coexpressing HEK 293 cells
| Construct | Time | ||
| 60 s | 240 s | 420 s | |
| GFP(± fsk, n.s.)a | 1.27 ± 0.09 | 1.28 ± 0.09 | 1.29 ± 0.11 |
| wtCFTR (no fsk) | 1.27 ± 0.15 | 1.36 ± 0.11 | ntb |
| wtCFTR(3 min fsk at 240 s) | 1.30 ± 0.05 | 0.98 ± 0.01c | 0.95 ± 0.01c |
| SLC26A9 + ΔF508CFTR(3 min fsk at 240 s) | 1.20 ± 0.05 | 1.20 ± 0.07 | 1.27 ± 0.08 |
a
GFP-alone constructs did not demonstrate significant differences in RIV in the presence or absence of forskolin stimulation, and the data are pooled.
b
nt, not tested.
c
P < 0.01 compared to same time point in other constructs.