Figure 1.

Western blot analysis for ezrin, radixin, and moesin in the adult and developing mouse testis. The cell lysates of the testes (Tes) from mice at 1–6 weeks and 10 weeks were electrophoresed in polyacrylamide gel, blotted onto polyvinylidene difluoride membrane, and stained with rabbit polyclonal anti-ezrin (H-276) antibody, rat monoclonal anti-radixin (R21) antibody, rat polyclonal anti-moesin antisera, or mouse monoclonal anti-α-tubulin antibody. For positive control, the same antibodies were applied to the cell lysates of the stomach (Sto), liver (Liv), and uterus (Ute) from adult mice. The molecular mass (kDa) of the immunoreactive bands is indicated.