Figure 4.

Immunoprecipitation (IP) analysis in the adult and 2-week mouse testes for the interaction of ezrin and radixin with β-actin and α-tubulin. (A) IP was performed in the cell lysate of the 10-week testis using rabbit polyclonal anti-ezrin antibody (H-276) or normal rabbit IgG. The testis lysate and products of IP were analyzed with Western blotting (WB) using anti-ezrin, anti-β-actin, or anti-α-tubulin antibody. (B) IP was performed in the cell lysate of the 2-week testis using rat monoclonal anti-radixin antibody or normal rat IgG. The testis lysate and products of IP were analyzed with WB using anti-radixin, anti-β-actin, or anti-α-tubulin antibody. The molecular mass (kDa) of the immunopositive bands is indicated.