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Merging of immunofluorescence images obtained from Foscan (0.3 mg/kg, IV)-photosensitized (10 J/cm2, 30 mW/cm2) HT29 subcutaneous xenografts frozen 24 hr after treatment. Frozen sections were first subjected to active caspase-3 (red fluorescence) and then to active caspase-7 (green fluorescence). Colocalization between both markers showed yellow spots (thick arrows). Red spots (thin arrows) indicate active caspase-3 expression only.
