Figure 1.

Conidiation rhythm in chol-1 cultures. Growth is from left to right. Bars = 24 h of growth. (A) Chol-1 strains on high choline. Cultures were grown in constant darkness on 100 μM choline. a, chol-1; b, chol-1 frq¹⁰; c, chol-1 wc-2. The chol-1 frq⁹ and chol-1 wc-1 strains were similar to chol-1 frq¹⁰ and chol-1 wc-2, respectively. This level of choline completely repairs the lipid synthesis defect in chol-1, and consequently chol-1 strains on high choline are indistinguishable from the corresponding chol⁺ strains on the same medium. (B) Effects of constant light on chol-1 strains. Cultures were grown on minimal medium without choline. a, c, e, g: Cultures were grown in constant darkness. b, d, f, h: Cultures were grown in constant light (cool-white fluorescent at approximately 20 μmol/m²/s). a, b, chol-1; c, d, chol-1 frq¹⁰; e, f, chol-1 wc-1; g, h, chol-1 wc-2.