Fig. 2.

Phenotype retention by Th1 and switching by Th17 cells in eyes of recipient mice. Polarized/reactivated Th1 (A) or Th17 (B, C) from 3A9 donors were injected into HEL-Tg mice and eyes of the recipients were collected 5 or 10 days later for intracellular staining of IFN-γ and IL-17. Th17 preparations were generated without (“I”) or with (“II”) anti-IL-2 Ab (see Materials and Methods for more detail). Left panels show representative experiments with the tested Th preparations, while the panels at the right summarize data of three repeated experiments with each of these preparations. The preparations used for the representative experiments contained 95% Th1, 60% Th17 “I” and 75% Th17 “II”. The summary columns are mean percent values ± SEM.