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. Author manuscript; available in PMC: 2009 Sep 30.
Published in final edited form as: Virology. 2008 Aug 6;379(2):197–204. doi: 10.1016/j.virol.2008.06.028

FIG. 3. Western blot analysis of US11 expression.

FIG. 3

MEF cells were either mock infected (lane 1), infected with KOS at an MOI of 10 (lanes 2–7) or infected with NM1 at an MOI of 10 (lanes 8–13) and harvested at 2, 4, 6, 9, 12, or 24 hours post infection, as indicated above the top panel. Proteins in the cell lysates were resolved by SDS-polyacrylamide gel electrophoresis and transferred to a PVDF membrane. The membrane was cut and the bottom half was probed using an antibody against US11 while the membrane from the top half was probed with an antibody against β-actin to confirm equal loading among the samples. Purified recombinant US11 was also tested as a positive control for the US11 antibody and for migration of the immunoreactive species (lanes 14 and 15).