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. Author manuscript; available in PMC: 2009 Apr 3.
Published in final edited form as: Mol Cell Neurosci. 2005 Sep;30(1):58–66. doi: 10.1016/j.mcn.2005.05.007

Fig. 5.

Fig. 5

Effect of gp120 and SDF-1α on the pro-apoptotic protein Apaf-1. The protein levels of Apaf-1 are increased in protein extracts obtained from gp120IIIB-treated (200 pM) neurons and immunoblotted with anti-Apaf-1 polyclonal antibody (A). No effect of gp120 on Apaf-1 was observed in the absence of glia using the same antibody (B). However, the Western blot analyses showed that Apaf-1 up-regulation by gp120 is blocked by the CXCR4 antagonist AMD3100 (100 ng/ml) (C). Treatment with AMD3100 also inhibits the increase in the level of active caspase 3 induced by gp120 (D), as assessed by immunoblots with antibodies against the cleaved (i.e. active) form of caspase 3. Both gp120 and SDF-1α (20 nM) increase p21 protein levels in neuronal protein extracts (E).