Figure 3.

PKA inhibition blocks Kv4.2myc activity-dependent internalization. A, Representative images showing internalized Kv4.2myc channels (green) and surface-remaining Kv4.2myc channels (red) after H89 (10 μm) pretreatment and AMPA (50 μm) stimulation in hippocampal neurons. B, Summary plot shows that the increase in intensity ratio (green integrated intensity/total integrated intensity) after AMPA treatment (n = 14) is prevented by pretreatment with H89 (n = 19). No differences (p > 0.05) were found between control (n = 23), H89 + AMPA (n = 19), and H89 alone (n = 13). All data are mean ± SE. *p < 0.001.