FIGURE 2.

LC/MS analysis of the reduction of DhtS catalyzed by human AKR1C isoforms. A, the ion chromatogram (m/z 350-400) of a mixture of authentic standards of DhtS and 3β-Diol-17-S; B-F, corresponding ion chromatograms of reaction samples containing no enzyme (B) and AKR1C1-4 (C-F). Samples were prepared as described under “Experimental Procedures.”