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LC/MS analysis of the reduction of TibS catalyzed by human AKR1C isoforms. A, the ion chromatogram (m/z 200-400) of a mixture of authentic standards of TibS, 3α-OH-TibS, and 3β-OH-TibS; B-F, corresponding ion chromatograms of reaction samples containing no enzyme (B) and AKR1C1-4 (C-F). Samples were prepared as described under “Experimental Procedures.”
