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Reactivity of the active site cysteines of sStoA. SDS-PAGE of purified reduced wild-type (wt) sStoA and C65A and C68A sStoA following reaction with MAL-PEG is shown. The protein variants were incubated with 0, 0.1, and 1 mm MAL-PEG before electrophoresis as indicated, and the gel was stained for protein. The lane indicated M contains molecular mass (kDa) markers.
