Skip to main content
. 2009 Apr 10;284(15):10150–10159. doi: 10.1074/jbc.M809509200

FIGURE 2.

FIGURE 2.

In vitro and in vivo characterization of thioredoxin Ile-75 variants. The variants are listed as shown in the bottom of each panel. A, redox potentials of thioredoxin and mutants except for the I75C and I75P mutants. Reduced I75C and I75P are too rapidly oxidized by air to allow for an accurate measurement of redox potential. B, in vitro reductase activity of thioredoxin and mutants, insulin reduction activity using DTT as the reducing source. C, in vitro reductase activity of thioredoxin and mutants, insulin reduction activity using NADPH with thioredoxin reductase as the reducing source. D, in vivo reductase activity of thioredoxin and mutants as measured by copper sensitivity. The gradient indicates a serial dilution of trxA null cells harboring thioredoxin and its variants (from 1 to 10-6 OD).