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Degradation profile of the RG chain. Profiles of the RG chain with YesW (A) YesX (C) and YesX del_loop mutant (B) were periodically analyzed by size exclusion chromatography. The reaction times were 0, 5, 15, 30, and 60 min. Product from RG chain without added enzyme was used as the negative control. All of the profiles are overlaid, and the peaks of tetrasaccharide and disaccharide are indicated by solid and dashed lines, respectively.
