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Transformation by v-Src requires annexin 2. Following exposure to either control or annexin 2 (anx 2) siRNA, R1LA29 cells were cultured in soft agar at both restrictive and permissive temperatures. The histogram shows the range of clump sizes according to the number of constituent cells. *, p = 0.023 (5-7 cells), 0.032 (8-16 cells), and 0.017 (17-25 cells); **, p = 0.0025 (1 cell) and 0.0003 (>25 cells). Error bars represent the S.E. of three different plates. RNAi, RNA interference.
