FIGURE 3.

Single channel recordings of purified MspA loop deletion mutants in lipid bilayers. Single-channel recordings of a diphytanoyl phosphatidylcholine membrane in the presence of 0.02, 500, or 0.1 ng/ml of wt, Δ3, and Δ5-Δ11 purified MspA protein, respectively. Protein solutions were added to both sides of the membrane, and data were collected from at least five different membranes. The membrane current was measured in an aqueous solution of 1 mKCl with an applied transmembrane potential of -10 mV.