TABLE 1.
Mutant | Plasmid | Primer pair for fragment A (5′ → 3′) | Primer pair for fragment B (5′ → 3′) |
---|---|---|---|
Δ3 | pML905 | TTCGGCCTGAACTCGGTCATCACC | GGTGATGTCACCGTCGTCGATCAGG |
CGACCAGCACGGCATACATC (psmyc1) | CGTTCTCGGCTCGATGATCC (pMS-seq1) | ||
Δ5 | pML906 | GGCCTGAACTCGGTCATCACCCCG | GATGTCACCGTCGTCGATCAGGATG |
CGACCAGCACGGCATACATC | CGTTCTCGGCTCGATGATCC | ||
Δ7 | pML907 | CTGAACTCGGTCATCACCCCGAACC | GTCACCGTCGTCGATCAGGATGTTC |
CGACCAGCACGGCATACATC | CGTTCTCGGCTCGATGATCC | ||
Δ9 | pML908 | AACTCGGTCATCACCCCGAACCTG | ACCGTCGTCGATCAGGATGTTCGG |
CGACCAGCACGGCATACATC | CGTTCTCGGCTCGATGATCC | ||
Δ11 | pML909 | TCGGTCATCACCCCGAACCTGTTC | GTCGTCGATCAGGATGTTCGG |
CGACCAGCACGGCATACATC | CGTTCTCGGCTCGATGATCC |