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Characterization of rLAP55 before and after protease treatment
| Treatment of rLAP55 | N terminus by Edman | Mass by TOF | Amino acids present | Mass by calculation | Enzyme activity after treatment |
|---|---|---|---|---|---|
| None | SEAQQF... | 54,923 | 27-536 | 54,925 | None |
| Trypsin | TPGKPN.. | 50,853 | 37-512 | 50,856 | ++++ |
| PA elastase | TPGKPN | 51,382 | 37-517 | 51,386 | ++++ |
| Chymotrypsin | TPGKPN | 51,498 | 37-518 | 51,499 | +++ |
| Chymotrypsin | TPGKPN | 53,671 | 37-536 | 53,671 | ?? |
| Neutrophil elastase | SEAQQF | 54,923 | 27-536 | 54,925 | +/− |
