TABLE 2.

The effects of IGF-1R mutation on the binding of inhibitory antibodies

a No effect (NE), measured K_D within 2.5-fold of WT hIGF-1R-Fc; weak (W), measured K_D between 2.5- and 10-fold higher than WT; medium (M), measured K_D between 10- and 100-fold higher than WT; strong (S), binding to antibody was ablated by mutation; and ND, not determined. DNE means did not express or expressed poorly because of intolerance of the protein fold for the mutation. M^*, measured >10-fold increase in affinity upon mutation. W^*, measured >5-fold increase in affinity upon mutation.