TABLE 3.

ITC-derived temperature dependence of IGF-1 and antibody binding to IGF-1R

Temperature	ΔHo	-TΔSo	ΔGo	Kd	Stoichiometry of binding (n)
°C	kcal/mol	kcal/mol	kcal/mol	nm
IGF-1
5	−16.4 ± 0.5	5.6	−10.8	3.3 ± 6.0	0.93
25	−26.8 ± 0.6	16.2	−10.6	19 ± 9	1.00
37a	−33.4 ± 0.5	23.6	−9.8	130 ± 60	1.02
BIIB5
5b	−11.3 ± 0.8				0.89
15b	−14.4 ± 0.6				0.98
25c	−20.1 ± 2.5	7.8d	−12.3d	1 ± 0.2d	0.98
37b	−29.2 ± 0.4				0.97
BIIB4
5b	−6.7 ± 1.0				0.90
25	−26.6 ± 0.6	15.1d	−11.5d	4 ± 0.5d	0.91
37b	−39.5 ± 1.2				0.70
BIIB3
25c	−14.1 ± 0.6				0.84

^aData are average of three experiments.

^bTight binding resulted in too few points in the transition region to measure K_d.

^cData are average of two experiments.

^dData were determined using both SPR and ITC experiments.