TABLE 4.

IGF-1/IGF-1R binding parameters determined at 25 °C in the absence and presence of saturated levels of inhibitory antibodies

IGF-1R antibody complex	ITC results					Equilibrium SPR results
	ΔH° ()	- T ΔS°	Δ G°	Kd	na	Kd	na
	kcal/mol	kcal/mol	kcal/mol	nm		nm
IGF-1R	− 26.8 ± 0.6	16.2	− 10.6	19 ± 9	1.00	11 ± 2	0.66
IGF-1R·BIIB5b	− 28.4 ± 2.0	17.9	− 10.5	20 ± 4	1.09	150 ± 15	0.65
IGF-1R·BIIB3b	− 21.7 ± 1.8	13.5	− 8.2	1000 ± 100	0.80	300 ± 40	0.5
IGF-1R·BIIB4c	-d	-	-	-	-	-	-

^aIGF-1·hIGF-1R-(1-903) binding stoichiometry.

^bApparent allosteric inhibitor.

^cApparent competitive inhibitor.

^dNo IGF-1 binding was observed.