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. 2009 Apr 10;284(15):10268–10275. doi: 10.1074/jbc.M900154200

FIGURE 4.

FIGURE 4.

SPR analysis of OASS-SAT interaction kinetics. a, SPR difference resonance signal of OASS binding to SAT monitored at varied concentrations of OASS (bottom to top, 0.018, 0.036, 0.18, 0.36, 0.72, 1.2 μm) as a function of time at 25 °C. RU, response units. b, kinetics of OASS (1.2 μm) binding to SAT. Fitting of the association data to a three-site model for the binding of three OASS dimers to one SAT trimer is shown as a gray line. The data fit to a one-site model is shown as the dashed line. c, kinetics of OASS (1.2 μm) dissociation from SAT. The gray line shows the fit of the data a three-site binding model. The data fit to a one-site model is shown as the black line.