TABLE 3.
Summary of kinetic parameters for OASS-SAT interaction determined by SPR
SPR experiments were performed as described under “Experimental Procedures.” Raw SPR data was fit to either a one-(0.018 and 0.036 μm OASS)-, two (0.18 and 0.36 μm OASS)-, or three (0.72 and 1.2 μm OASS)-site binding model to obtain values for each association and dissociation event.
| [OASS] | Kd,1 | Kd,2 | Kd,3 | ka,1 | ka,2 | ka,3 |
|---|---|---|---|---|---|---|
| μm | ×10−3 s−1 | ×10−4 s−1 | ×10−5 s−1 | ×105m−1 s−1 | ×104m−1 s−1 | ×103m−1 s−1 |
| 0.018 | 2.2 ± 0.02 | 2.5 ± 0.01 | ||||
| 0.036 | 1.9 ± 0.02 | 1.4 ± 0.01 | ||||
| 0.180 | 6.0 ± 0.03 | 2.0 ± 0.02 | 2.0 ± 0.01 | 6.2 ± 0.01 | ||
| 0.36 | 2.1 ± 0.01 | 1.9 ± 0.01 | 2.5 ± 0.01 | 4.1 ± 0.01 | ||
| 0.72 | 2.3 ± 0.01 | 2.1 ± 0.02 | 4.3 ± 0.06 | 2.5 ± 0.02 | 3.9 ± 0.01 | 6.3 ± 0.02 |
| 1.2 | 1.9 ± 0.01 | 2.4 ± 0.03 | 4.4 ± 0.04 | 2.5 ± 0.01 | 2.0 ± 0.01 | 2.6 ± 0.02 |