TABLE 5.
Evaluation of alternatively TilS-modified tRNAs from Table 4 as substrates for tRNA synthetases
|
tRNA substrate (amine modifier)
|
Relative aminoacylation
ratea
|
||||
|---|---|---|---|---|---|
| E. coli MetRS | E. coli IleRS | B. subtilis IleRS | |||
| % | |||||
| tRNAMet | (100) | 7 | 32 | ||
| Reaction A (control) | 23 | 2 | 0 | ||
| Reaction B (lysine) | 0 | (100) | (100) | ||
| Reaction C (aminoethylcysteine) | 1 | 104 | 127 | ||
| Reaction D (aminoethylserine) | 0 | 44 | 95 | ||
| Reaction E ((5R)-5-hydroxylysine) | 4 | 23 | 130 | ||
| Reaction F (cadaverine) | 1 | 356 | 133 | ||
| Reaction G (4,5-trans-dehydrolysine) | 2 | 19 | 105 | ||
| Reaction H (ethanolamine) | 0 | 123 | 99 | ||
| Reaction I (N-acetylethylenediamine) | 3 | 31 | 92 | ||
| Reaction J (histamine) | 0 | 205 | 116 | ||
a
The 100% reference corresponds to 3240, 2210, or 4600 net cpm for E. coli MetRS, E. coli IleRS, or B. subtilis IleRS, respectively, measured after a 2-h assay as described under “Experimental Procedures”.