FIGURE 4.

Overexpression of cyclophilin D inhibits apoptosis in a CsA- and Bcl2-dependent manner. HEK293T cells, stably transfected with the control empty vector or with the CypD expression vector, were treated with etoposide at 20 μm for 6 h in the absence or presence of CsA at 1 μm. A subset of cells was co-transfected with the pKD-Bcl2 shRNA vector to achieve knockdown (KD) of Bcl2 and also treated with etoposide. Apoptotic cells with condensed nuclei were detected using the Hoechst 33342 nuclear stain. Data are means ± S.D. (n = 3). * indicates p < 0.05 when compared with EV controls.