TABLE 1.
Initial acyl substrate screen
| Activitya | |
|---|---|
| nmol/min/mg | |
| Phytohormones | |
| Indole-3-acetate | 1.5 ± 0.1 |
| Jasmonate | 2.9 ± 0.1 |
| Gibberellate | 0.1 ± 0.1 |
| Abscisate | 0.8 ± 0.1 |
| Salicylate | 1.7 ± 0.1 |
| INA | 0.1 ± 0.2 |
| Benzoates | |
| Benzoate | 59.6 ± 1.1 |
| Phenylacetate | 2.3 ± 0.1 |
| Salicylate (2-hydroxybenzoate) | 1.7 ± 0.1 |
| 4-Hydroxybenzoate | 135.2 ± 8.6 |
| Chorismate pathway compounds | |
| Chorismateb | 32.9 ± 2.0 |
| Prephenate | 10.1 ± 0.4 |
| Anthranilate (2-aminobenzoate) | 0.7 ± 1.1 |
| Phenylalanine | 0.0 ± 0.0 |
| Tyrosine | 0.0 ± 0.0 |
| Tryptophan | 0.0 ± 0.0 |
| trans-Cinnamate | 21.3 ± 1.8 |
a
Activities are the mean of three replicates ± 1 S.D. and are determined for 1 mm acyl substrate using the coupled adenylation assay. Activities below 10 nmol/min/mg are minimal. Additional experiments yielded similar results.
b
Activity is due to 4-HBA in chorismate preparation.