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Kinetic parameters of PBS3 substrates
Kinetic constants were determined by following acyl substrate-Glu formation by HPLC. Similar results were obtained for experiments with an independent enzyme preparation.
| Acyl substrate | kcat | Km | kcat/Km |
|---|---|---|---|
| min-1 | μm | min-1 μm-1 | |
| 4-HBA | 20.36 | 459 | 0.0444 |
| pABA | 2.74 | 153 | 0.0179 |
| BA | 3.96 | 867 | 0.0046 |
