TABLE 4.
Fractional inhibition of pABA-AMP formation
| Compound | Fractional inhibitiona(30 μm) | Fractional inhibition (300 μm) |
|---|---|---|
| SA | 0.33 | 0.75 |
| MeSA | 0.02 | 0.00 |
| INA | 0.09 | 0.70 |
| IAA | 0.04 | 0.19 |
| JA | 0.02 | 0.10 |
| 3-HBA | 0.04 | 0.22 |
a
Fractional inhibition compares activity with additional compound to control reaction. Inhibition was assessed using the coupled adenylation assay with 150 μm pABA as the acyl substrate and 30 or 300 μm of the compound being tested. Repeat experiments with a separate enzyme batch gave similar results.