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TGFβ-induced Smad1 phosphorylation is observed in Smad3 null MEF cells. TGFβ-induced phospho-Smad1 is present in Smad3 null MEF cells. Wild type (WT) and Smad3 null (Smad3–/–) MEF cells were cultured in the presence or absence of TGFβ or BMP for 1 h and harvested for Western blot analysis with anti-Smad and anti-phospho-Smad antibodies. IB, immunoblot.
