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. 2009 Apr 10;284(15):9755–9763. doi: 10.1074/jbc.M809223200

FIGURE 7.

FIGURE 7.

TGFβ-induced Smad1 phosphorylation is independent of BMP type I receptors in certain cell lines. A, C2C12 cells were co-treated with ligand (TGFβ or BMP) and ALK inhibitor (SB431542 or DM), as indicated, for 1.5 h prior to harvest for Western blot analysis as described in the legend to Fig. 1A. B, HepG2 cells were co-treated with ligand (TGFβ or BMP) and ALK inhibitor (SB431542 or DM), as indicated, for 1.5 h prior to harvest for Western blot analysis as described in the legend to Fig. 1A. C, MEF cells were co-treated with ligand (TGFβ or BMP) and ALK inhibitor (SB431542 or DM), as indicated, for 1.5 h prior to harvest for Western blot analysis as described in the legend to Fig. 1A. D, HaCaT cells were co-treated with ligand (TGFβ or BMP) and ALK inhibitor (SB431542 or DM), as indicated, for 1.5 h prior to harvest for Western blot analysis as described in the legend to Fig. 1A. IB, immunoblot.