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. 2009 Apr 10;284(15):9755–9763. doi: 10.1074/jbc.M809223200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — TGFβ promotes Smad1 phosphorylation independently of ALK1/2/3/6 in certain cell lines. In C2C12 and HepG2 cells (left panel) TGFβ-induced Smad1/2/3 phosphorylation can be blocked by SB431542, suggesting these phosphorylation events depend on ALK4/5/7 activity. However, these phosphorylation events appear to be independent of ALK1/2/3/6 activity because they are not blocked by DM. As expected, BMP-induced Smad1 phosphorylation is blocked by DM in C2C12 and HepG2 cells. In HaCaT cells (right panel) TGFβ-induced Smad1/2/3 phosphorylation can also be blocked by SB431542, suggesting these events also depend on ALK4/5/7 activity. However, in contrast to C2C12 and HepG2 cells, both TGFβ and BMP-induced Smad1 phosphorylation can be blocked by DM, suggesting that both events depend on ALK1/2/3/6 activity in HaCaT cells.