FIGURE 3.

Analysis for O-sulfotransferase activity of DS-epi2. Microsomes were prepared from 293HEK cells overexpressing full-length DS-epi1 or DS-epi2 and resuspended in dialysis buffer without detergent. A, Western blot of 25 μg of microsomal preparations. B, O-sulfotransferase activity was assayed (mean ± 2 S.D. of triplicates) without detergent (black bars) or with detergent (empty bars). The O-sulfotransferase assay shown was performed at pH 6.8 using chondroitin as substrate. Twenty-five μg of microsomes from mock cells incorporated 2,700 dpm, when assayed with detergent.