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. 2009 Apr 10;284(15):9845–9853. doi: 10.1074/jbc.M809272200

FIGURE 6.

FIGURE 6.

NHEJ activity is lower in U2OS-PO and -PS cells than in U2OS cells. A, map of the pEGFP-Pem1-Ad2 vector. Note the Pem1 intron, Ad2 exon, and location of the HindIII excision sites. B, dot plots of untransfected U2OS cells (panel 1), U2OS cells co-transfected with pEGFP-Pem1 and DsRed (panel 2), U2OS cells co-transfected with HindIII-linearized pEGFP-Pem1-Ad2 and DsRed (panel 3), U2OS-PO cells co-transfected with HindIII-linearized pEGFP-Pem1-Ad2 and DsRed (panel 4), and U2OS-PS cells co-transfected with HindIII-linearized pEGFP-Pem1-Ad2 and DsRed (panel 5). The numbers of green (green fluorescent protein) and red (DsRed) cells were determined by FACS analysis, and typical FACS traces are shown. The ratio of green fluorescent protein to DsRed was used as a measure of NHEJ efficiency. All measurements of the rejoining efficiency were carried out 24 h after transfection with the corresponding plasmids. C, percentage of ligated DNA ends was determined by using the data in B. All experiments were repeated at least four times, and the S.D. are shown.