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The R117Q mutation resulted in defective embryonic development. A, FADD+/– mice were crossed with FADD+/– R117Q:GFP mice. Pregnancy was timed, and embryos of various developmental stages were isolated for stereomicroscopic analysis. Genotypes of embryos were determined by Southern blotting (B) and PCR (C), proteins were extracted from whole embryos, and wild type and mutant FADD:GFP proteins expression in embryos were confirmed by Western blotting (D). K/O, knock out.
