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. 2009 Apr 10;284(15):9937–9946. doi: 10.1074/jbc.M900719200

FIGURE 2.

FIGURE 2.

Steady-state kinetic analyses of KshAB-catalyzed transformations. Shown is the dependence of the initial velocity of O2 consumption on ADD (A) and AD (B) concentrations in air-saturated buffer with fitted parameters Km = 110 ± 20 μm and 24 ± 16 μm, and Vmax = 0.32 ± 0.02 μm s–1 and 0.032 ± 0.006 μm s–1, respectively. C, dependence of the initial velocity of O2 consumption on O2 concentration in the presence of 380 μm ADD. The best fit of the Michaelis-Menten equation to the data using the least squares dynamic weighting options of LEONORA is represented as a solid line.