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Apparent steady-state kinetic parameters of KshAB of M. tuberculosis
Experiments were performed using 0.1 m potassium phosphate, pH 7.0, at 25 °C. Values reported in parentheses represent S.E.
| Substrate | kcat | Km | kcat/Km |
|---|---|---|---|
| s-1 | μm | m-1s-1 | |
| ADD | 0.80 (0.05) | 110 (20) | 7600 (700) |
| AD | 0.07 (0.01) | 24 (16) | 3000 (2000) |
| O2a | >7 | >1200 | 2450 (80) |
Determined in the presence of 380 μm ADD.
