Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Apr 10;284(15):9965–9973. doi: 10.1074/jbc.M807442200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Protein interactions of OCRL1 clathrin box mutants. A and B, GFP-tagged OCRL1 isoform a or b wild type (WT) or the indicated clathrin box mutants were expressed in HeLa cells and tested for binding to GST-tagged bait proteins as indicated. Bound proteins were detected by Western blotting with anti-OCRL1 antibodies. C, OCRL1 isoform a or b or the indicated mutants were tested for interaction with clathrin terminal domain (CTD) or the α-adaptin appendage domain (α-ear) in the yeast two-hybrid system. Growth on high selection indicates interaction. D, in vitro synthesized wild-type OCRL1 isoform a or the indicated mutants were tested for binding to the indicated GST-tagged bait proteins. Bound OCRL1 was detected by autoradiography. Substituted residues are indicated in bold.