Table 1. Circadian gene expression in rhythmic and arrhythmic lungs.

mRNA levels of circadian clock related genes were compared between lung tissue at P3 (n=7) and P30 (n=4). Tissues harvested at ZT 0 and 12 were averaged and mean and SEM were plotted. No statistical differences (by t-test) were observed in the expression levels of Per1, Per2, Per3, Cry1, Cry2, Bmal1, Clock, NPAS2, Ck1ε and Tim.

Result of quantitative RT-PCR analysis.

	Postnatal day 3	Adult	p value
Per1	1.65 ± 0.09	1.83 ± 0.36	0.55
Per2	2.38 ± 0.09	2.77 ± 0.37	0.22
Per3	0.15 ± 0.02	0.23 ± 0.04	0.07
Cry1	1.10 ± 0.17	1.25 ± 0.28	0.64
Cry2	0.05 ± 0.01	0.07 ± 0.02	0.43
Bmal1	1.01 ± 0.13	0.58 ± 0.16	0.06
Clock	0.15 ± 0.02	0.23 ± 0.04	0.07
NPAS2	0.08 ± 0.02	0.09 ± 0.04	0.83
Ck1ε	1.05 ± 0.16	1.42 ± 0.22	0.20
Tim	0.90 ± 0.09	0.72 ± 0.02	0.19