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. Author manuscript; available in PMC: 2009 Apr 3.
Published in final edited form as: Hear Res. 2007 Jun 27;232(1-2):1–19. doi: 10.1016/j.heares.2007.06.011

Figure 6.

Figure 6

At low magnification, phalloidin-labeled explants treated with actin disruption agents – cytochalasin D (A) and Latrunculin A (E) contain fewer missing hair bundles compared to explants treated with both gentamicin and actin disruption agents only (B,F). High magnification imaging of regions containing missing hair cells (C,D,G,H) show dark circular or ovoid areas in the sensory epithelium. (C) A projection of F-actin phalloidin labeling of hair cell lesions in a cytochalasin D-treated explant. (D) A single optical section from the projection shown in (D), with intense fluorescence for F-actin (arrows) at the junctional confluences between two supporting cells (arrows in C). (G) Projection of hair cell lesions in latrunculin A-treated explants. (H) A single optical section from the projection shown in (G), that does not show intense F-actin phalloidin labeling (arrows) at the junctional confluences between two supporting cells (arrows in G).