Figure 3.

rAAVCre-transduced cells persist long-term in the mouse lung. (a) Whole-mount X-gal staining of Rosa26-Flox/LacZ mouse lungs infected with 2 × 10¹⁰ particles of rAAV1Cre or rAAV5Cre virus at 1 week, 2 weeks, 1 month, 3 months, and 6 months after infection. (b) Quantification of β-galactosidase activity from Rosa26-Flox/LacZ mouse lungs infected with rAAVCre viruses. rAAV expression values shown are relative to β-galactosidase activity from Rosa26-LacZ mouse lungs at each time point. Data represent the mean (±SEM) relative β-galactosidase activity per mg/protein from three mice per experimental point (three independent experiments).