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. Author manuscript; available in PMC: 2009 Apr 3.
Published in final edited form as: J Invest Dermatol. 2008 Oct 16;129(4):843–850. doi: 10.1038/jid.2008.320

Figure 7. Effects of Ets-1 on collagen expression inhibited by LL-37.

Figure 7

(a) HDF cells were harvested after LL-37 (40 nm) treatment for 24 h. Total RNA was extracted, and cDNA was synthesized for RT-PCR. (b) Cells were transfected with siEts-1. After 24 hours, total RNA was extracted, and cDNA was synthesized for RT-PCR. The histogram shows Ets-1 mRNA level relative to β-actin by densitometry. Cells were transfected with control siRNA or siEts-1. Cells were harvested after LL-37 treatment for 24 or 48 h. (c) After 24 hours, collagen gene expression was detected by real-time RT-PCR. Bars indicate mean±SD of three independent experiments, each with triplicate samples. (d) After 48 hours cells were harvested, type I collagen production was analyzed by Western blot. The band intensities were quantitated.