Skip to main content
. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Cancer Res. 2009 Feb 24;69(5):1985–1994. doi: 10.1158/0008-5472.CAN-08-3934

Figure 5. Bmf or Bim RNAi protects melanoma cells from CI-1040 induced apoptosis.

Figure 5

(A) Following stable lentiviral shRNA expression targeting Bim, Bmf, or scrambled control (Ctrl) in the sensitive M14-MEL cell line, cells were treated with 2μM CI-1040 or equal volume DMSO for 72 h and apoptosis (% sub G1 fraction) was determined by flow cytometry. Data is expressed as the mean ± SEM. Two different shRNAs with different efficiencies were used. Reduced expression of Bim and Bmf was confirmed by Western blot analysis with the antibodies indicated to the left of each panel. Blots were stripped and reprobed with α-Tubulin as a loading control. (B) Apoptotic sensitivity of M14-MEL cells treated with 2μM CI-1040 and increasing levels of the pan caspase inhibitor ZVAD-FMK (○), the caspase 3 inhibitor Z-DEVD-FMK (▲), or the caspase 8 inhibitor Z-IETD-FMK(◇). Apoptosis was determined as described in (A). Data is expressed as the mean ± SEM.