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. 2009 Feb 2;37(6):1843–1853. doi: 10.1093/nar/gkn1049

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Sem1p and Ubp6p participate in telomeric silencing. (A, B) Sem1p and Ubp6p are required for telomeric silencing. (C) Loss of Ubp6p rescues silencing defects in the ubp10Δ mutant. (D) Sem1p and Ubp6p do not play a direct role in HM and rDNA silencing. Modified hmrΔE locus and rDNA array in CCFY101α were shown on the top. Sensitivity to 5-FOA was performed by spotting 10-fold serial dilutions of cells onto synthetic complete (SC) plates and synthetic complete plates containing 0.1% 5-FOA (SC+FOA), respectively. The red/white colony color assay was conducted by re-streaking cells on YPD plates. Plates were photographed after 5 to 7 days of growth at 30°C. Genotypes of yeast strains are indicated on the left. Assays were repeated three times. WT, wild-type.