Figure 5.

Sem1p and Ubp6p are involved in the proteolytic function of the proteasome. (A) Sensitivity to canavanine was done by spotting 10-fold serial dilutions of cells onto synthetic complete plates with arginine dropped out (SC-ARG) and SC-ARG plates containing 1 μg/ml canavanine (SC-ARG+CAN). Plates were photographed after 3–5-day growth at 30°C. Genotypes of yeast strains are indicated at the left. (A) Sem1p and Ubp6p regulate telomeric silencing independently of the proteolytic function of the proteasome. Indicated strains were treated with MG-132 (50 μM) or DMSO for 30 min and processed for RT-PCR All assays were repeated three times. WT, wild-type.