Table 2.

Characterization of the transformants of the wild-type strain with integrative plasmid YIplac204-LPG carrying either undamaged or MDS-1-containing oligonucleotide

Transformation with YIplac204-LPG carrying oligonucleotide		Undamaged	MDS-containing	Controla
Frequency of Trp+Leu+ clones observed in two experimentsb	I	6.1 × 10−6	7.2 × 10−6	2.6 × 10−6
	II	10 × 10−6	7.6 × 10−6	3.8 × 10−6
Total clones analyzed		26	39
Total integrations into trp1 locus		8 (31%)	16 (41%)
Integrations into trp1 locus those that contain oligonucleotide		8 (100%)c	16 (100%)c
Total integrations outside of trp1 locus		18 (69%)d	23 (59%)e
Integrations outside of trp1 locus those that contain oligonucleotide		14 (78%)	18 (78%)
Total clones carrying oligonucleotide		22 (85%)	34 (87%)	NAf

^aTransformation with NheI/XhoI/BsgI-digested YIplac204-LPG (see Materials and methods section).

^bThe mean RTE (see footnote to Table 1) from the two experiments is 0.98.

^cAll samples yielded PCR product with either ES3/trp-R primers or gal-P/trp-R primers.

^dThree samples yielded PCR product neither with ES3/trp-P primers, nor with gal-P/trp-P primers. One sample yielded PCR product with gal-P/trp-P primers, but not with ES3/trp-P primers.

^eFour samples yielded PCR product neither with ES3/trp-P primers, nor with gal-P/trp-P primers. One sample yielded PCR product with gal-P/trp-P primers, but not with ES3/trp-P primers.

^fTransformants were not analyzed by PCR.