Table 1.

Mutations identified during the screening and further analyzed

Sup45 allele	Amino-acid substitution	Nucleotide change	Comments
C11	N58K	AAT⇨AAA	NIKS motif in the domain 1
B1	Q76R	CAA⇨CGA	P2
C6	Q76K	CAA⇨AAA	P2
A8	E104K	GAA⇨AAA	P1
A2	E360V	GAA⇨GTA	Only domain 3
B3	E360G	GAA⇨GGA	Only domain 3
A4	N27H, K331N	AAT⇨CAT, AAA⇨AAT	P1
A5	Q46K, K115R	CAA⇨AAA, AAA⇨AGA	P2
A19	T50K, N379Y	ACA⇨AAA, GTT⇨GTA	P2
A13	Q76K, K340E	CAA⇨AAA, AAA⇨GAA	P2
C1	D96E, E360G	GAT⇨GAA, GAA⇨GGA	P1
A16	K276E, E345V	AAG⇨GAG, GAA⇨GTA	Only domain 3
C12	L21I, N209K, E315V	ATA⇨TTA, AAT⇨AAA, GAA⇨GTA	Domains 2 + 3
A18	D96E, N161I, I211V	GAT⇨GAA, AAT⇨ATT, ATT⇨GTT	P1
C15	E104K, C299S, I326N	GAA⇨AAA, TGT⇨AGT, ATC⇨AAC	P1
C3	D149N, Y287S, I387V	GAC⇨AAC, TAT⇨TCT, ATC⇨GTC	Domains 2 + 3
C2	I248V, E360G, I387T	ATT⇨GTT, GAA⇨GGA, ATC⇨ACC	Only domain 3

The various types of nucleotide transition and transversion are indicated, together with the resulting amino-acid changes. Mutants are listed in order of the corresponding mutations, from N-terminus to C-terminus residues. The column ‘comments’ correspond to the annotation of the bold residue (if more than one mutation) indicated in the second column. The complete list of mutations identified during the screening can be found in Supplementary Table SII.