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. 2009 Feb 10;37(6):1973–1983. doi: 10.1093/nar/gkp027

Figure 2.

Figure 2.

Definition of the EbfC preferred binding sequence. (A) Representative EMSA of DNA probes containing two, one or no EbfC-binding consensus sequences and titration of increasing amounts of EbfC. Lanes 1–3 contain probe b-WT, lanes 4–6 contain probe b-C1, lanes 7–9 contain probe b-C2 and lanes 10–12 contain probe b-C1/2. Lanes 1, 4, 7 and 10 lacked EbfC, lanes 2, 5, 8 and 11 contained 8 μM EbfC and lanes 3, 6, 9 and 12 contained 40 μM EbfC. (B) Association of 4 μM EbfC with probes b-WT (lane 2), b-C1 (lane 3), b-C2 (lane 4), b-C1/2 (lane 5) and b-C2 derivatives b-C20 (lane 6), b-C30 (lane 7), b-C40 (lane 8) and b-C50 (lane 9). (C) Definition of the EbfC high-affinity binding sequence. Lane numbers refer to the EMSA lanes shown in (B).